Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XI. Characterization of selectively deuterated analogs of staphylococcal nuclease.
نویسندگان
چکیده
The preparation of five different selectively deuterated analogs of the enzyme staphylococcal nuclease has been previously reported.(1-3) The five selectively deuterated enzymes have full enzymatic activity with DNA and RNA substrates and identical amino acid compositions; and they all appear to have similar conformations in solution, despite their very different hydrogen/deuterium contents.
منابع مشابه
Nuclear magnetic resonance studies of the structure and binding sites of enzymes. XII. A conformational equilibrium in staphylococcal nuclease involving a histidine residue.
Evidence is presented for the existence of a conformational equilibrium in staphylococcal nuclease, involving one out of the four histidines and none of the other aromatic residues in the polypeptide chain.
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ورودعنوان ژورنال:
- Proceedings of the National Academy of Sciences of the United States of America
دوره 65 2 شماره
صفحات -
تاریخ انتشار 1970